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Jena Bioscience
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End, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3+end/5'-bisphosphate-Cy5/bio_rxiv__64898__2026__05__28__728402-82-6-25
Average 95 stars, based on 7 article reviews
End, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3+end/5'-bisphosphate-Cy5/bio_rxiv__64898__2026__05__28__728402-82-6-25
Average 95 stars, based on 7 article reviews
end - by Bioz Stars,
2026-10
95/100 stars
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Figure Legend Snippet: A) Fluorescence microscopy images of preassembled equimolar condensates showing reciprocal recruitment of HOTAIR 1-360 and AR LBD . Upper: DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after addition of 25 μM Cy3-labeled HOTAIR 1-360 . Lower: DyLight488-labeled AR NTD and Cy3-labeled HOTAIR 1-360 droplets after addition of 20 μM Cy5-labeled AR LBD . An equimolar mixture of Cy5-labeled AR LBD and Cy3-labeled HOTAIR 1-360 (25 μM each) did not undergo LLPS. Scale bar, 10 μm. Representative of three biological replicates. B) Quantification of Cy3-labeled HOTAIR 1-360 enrichment in preassembled AR NTD -HOTAIR 1-360 droplets before and after the addition of 20 μM Cy5-labeled AR LBD . C) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of 25 μM Cy3-labeled HOTAIR 1-360 . D) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of an equivalent amount of PolyU-20nt. For B-D, enrichment was calculated from raw-image fluorescence measurements. Boxes indicate quartiles and the mean; dots represent individual droplets across three biological replicates. Representative images are shown with identical linear scaling within each channel across conditions. E-G) Fluorescence microscopy images from three biological replicates of preassembled equimolar DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after the addition of E) 400 μM SLNCR1-20nt, F) HOTAIR-20nt, or G) PolyU-20nt. Scale bar, 10 μm. The corresponding normalized line-scan intensity profiles are shown.
Techniques Used: Fluorescence, Microscopy, Labeling
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Labeling:Article Title: LLPS REDIFINE allows the biophysical characterization of multicomponent condensates without tags or labels. Article Snippet: .. S2m RNA was 3’end labeled with Article Title: LLPS REDIFINE allows the biophysical characterization of multicomponent condensates without tags or labels Article Snippet: .. S2m RNA was 3’end labeled with other:Article Title: Binding of long non-coding RNAs within the androgen receptor N-terminal tail modulates interdomain communication and liquid-liquid phase separation Article Snippet: Short RNA oligonucleotides (SLNCR1-20nt, HOTAIR-20nt, PolyU-20nt, PolyA-20nt, RNA-NC-20nt) carrying Cy5 label at the 3’ end were ordered from Eurofins Genomics. Incubation:Article Title: Dynamics of microcompartment formation at the mitosis-to-G1 transition Article Snippet: First, digested chromatin was 5’ phosphorylated in end-repair reactions (50 U T4 Polynucleotide Kinase (New England BioLabs #M0201), 50 mM NaCl, 10 mM Tris-HCl pH = 7.5, 10 mM MgCl2, 100 ug/mL BSA, 2 mM ATP (ThermoFisher #R1441), 5 mM DTT (Sigma-Aldrich #10197777001), in water) at 37°C for 15 min while mixing. .. Next, 50 U of DNA Polymerase I Klenow Fragment (New England BioLabs #M0210) was added to the reaction and incubated at 37°C for 15 min while mixing to create 5’ fragment overhangs, and these overhangs were filled in by adding a mixture of dNTPs in |